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cd163 polyclonal antibody  (Proteintech)


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    Proteintech cd163 polyclonal antibody
    Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of <t>CD163</t> in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).
    Cd163 Polyclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cd163 polyclonal antibody/product/Proteintech
    Average 96 stars, based on 282 article reviews
    cd163 polyclonal antibody - by Bioz Stars, 2026-03
    96/100 stars

    Images

    1) Product Images from "Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration"

    Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2025.11.009

    Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).
    Figure Legend Snippet: Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Techniques Used: Staining, Fluorescence, Cell Culture, Marker, Flow Cytometry, Labeling

    Evaluation of immune inflammation (BMDM) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in BMDM cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in BMDM cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).
    Figure Legend Snippet: Evaluation of immune inflammation (BMDM) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in BMDM cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in BMDM cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Techniques Used: Staining, Fluorescence, Cell Culture, Marker



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    Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of <t>CD163</t> in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).
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    Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of <t>CD163</t> in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).
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    Image Search Results


    Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Journal: Bioactive Materials

    Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration

    doi: 10.1016/j.bioactmat.2025.11.009

    Figure Lengend Snippet: Evaluation of immune inflammation (RAW264.7) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD206 in RAW264.7 cells co-cultured with different sample extracts, with CD206 (M2 marker) shown in red (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in RAW264.7 cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). E, F) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in RAW264.7 cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). G) Flow cytometry analysis of CD86 and CD11b positive cells in inflammatory RAW264.7 cells. H) Fluorescence enzyme labeling for the quantitative analysis of ROS content in macrophages. I) Cell viability of RAW264.7 cells co-cultured with different sample extracts for 1, 3, and 5 days. J) NO content in RAW264.7 cells co-cultured with different sample extracts. K) DCFH-DA fluorescent probe detection to evaluate ROS content in macrophages after stimulation with different sample extracts (scale bar = 100 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Article Snippet: Following stimulation, the cells were blocked with 5 % bovine serum albumin (BSA) for 2 h at room temperature, washed three times with PBS, and incubated overnight at 4 °C with a CD86 polyclonal antibody (1:600, 13395-1-AP, Proteintech, USA) and CD163 polyclonal antibody (1:600, 16646-1-AP, Proteintech, USA).

    Techniques: Staining, Fluorescence, Cell Culture, Marker, Flow Cytometry, Labeling

    Evaluation of immune inflammation (BMDM) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in BMDM cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in BMDM cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Journal: Bioactive Materials

    Article Title: Immune regulative GelMA&Zn 2+ /Ce 3+ -whitlockite scaffolds with continuous ions release for bone regeneration

    doi: 10.1016/j.bioactmat.2025.11.009

    Figure Lengend Snippet: Evaluation of immune inflammation (BMDM) in the scaffolds. A, B) Immunofluorescent staining results and quantitative fluorescence intensity of CD86 in BMDM cells co-cultured with different sample extracts, with CD86 (M1 marker) shown in green (scale bar = 50 μm). C, D) Immunofluorescent staining results and quantitative fluorescence intensity of CD163 in BMDM cells co-cultured with different sample extracts, with CD163 (M2 marker) shown in green (scale bar = 50 μm). P values were determined using one-way ANOVA (∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01, ∗P < 0.05, N.S., not significant).

    Article Snippet: Following stimulation, the cells were blocked with 5 % bovine serum albumin (BSA) for 2 h at room temperature, washed three times with PBS, and incubated overnight at 4 °C with a CD86 polyclonal antibody (1:600, 13395-1-AP, Proteintech, USA) and CD163 polyclonal antibody (1:600, 16646-1-AP, Proteintech, USA).

    Techniques: Staining, Fluorescence, Cell Culture, Marker